Description
Principle of the Assay: Caspase (Cysteine-requiring Aspartate Protease) is a protease family that plays an important role in the process of apoptosis. Caspase 8 (also known as FLICE, MACH or mch5) is considered to be a caspase in the upstream of apoptotic signal transduction. Caspase 8 is activated in Fas-receptor and TNFR-1-mediated apoptotic process, forming a dimer composed of P18 and p10, which further activates downstream Caspase 4, Caspase 6, Caspase 9 and Caspase 10. Caspase 8 exists in the form of prozyme in the normal state and has no activity. However, Caspase 8 is activated in the apoptotic stage and participates in the apoptotic process. This kit is used to conjugate Caspase 8 sequence-specific peptides acetyl-Ile-Glu-Val-Asp p-nitroanilide (AcIETD-pNA) to yellow group p-nitroaniline (pNA). When the substrate is cut by Caspase 8, the yellow group pNA is dissociated. pNA has an absorption peak at 405nm. Measure the OD value at 405 nm and then Caspase 8 activity can be calculated accordingly: